Lesbophobia (sometimes lesbiphobia) comprises various forms of negativity towards lesbians as individuals, as couples, or as a social group. Based on the categories of sex, sexual orientation, lesbian identity, and gender expression, this negativity encompasses prejudice, discrimination, and abuse, in addition to attitudes and feelings ranging from disdain to hostility. As such, lesbophobia is sexism against women that intersects with homophobia and vice versa.

While some people use only the more general term "homophobia" to describe this sort of prejudice or behavior, others believe that the terms "homosexual" and "homophobia" do not adequately reflect the specific concerns of lesbians, because they experience the double discrimination of both homophobia and sexism. Similarly, bisexual women may prefer to use the term biphobia to refer to prejudice or abuse that they encounter which is based on their bisexual identity or behaviour, and people who identify as transgender often prefer to use the word transphobia.