Biphobia is aversion toward bisexuality and toward bisexual people as a social group or as individuals. It can take the form of denial that bisexuality is a genuine sexual orientation, or of negative stereotypes about people who are bisexual (such as the beliefs that they are promiscuous or dishonest). People of any sexual orientation can experience or perpetuate biphobia, and it is a source of social discrimination against bisexual people.

Lesbophobia (sometimes lesbiphobia) comprises various forms of negativity towards lesbians as individuals, as couples, or as a social group. Based on the categories of sex, sexual orientation, lesbian identity, and gender expression, this negativity encompasses prejudice, discrimination, and abuse, in addition to attitudes and feelings ranging from disdain to hostility. As such, lesbophobia is sexism against women that intersects with homophobia and vice versa.

During the year prior to the 2015 U.S. survey, 59 percent of respondents reported avoiding using a public restroom out of fear of violence or harassment. 32 percent limited the amount they ate or drink in order to avoid using a public restroom. Eight percent reported suffering a urinary tract infection, kidney infection, or other kidney problem as a result of avoiding public restrooms.

Thirty-three percent reported having negative experiences with a healthcare professional related to being transgender, such as verbal harassment or denial of treatment. 23 percent reported that they did not seek treatment for a condition out of fear of being mistreated, while 33 percent did not seek treatment because they were unable to afford it.

During the month prior to the survey, 39 percent of American transgender people experienced major psychological distress, compared to 5 percent of the general population of the United States. 40 percent had attempted suicide at some point in their life, compared to 4.6 percent of the American population. Family and community support were correlated with far lower rates of suicide attempts and of major psychological distress.

A study conducted on transgender women of color in San Francisco has shown a higher correlation between transphobia and risk of transgender women engaging in HIV risk behavior. The study shows that the transgender youth face social discrimination, and they may not have a social role model. The young adults in this group have shown a higher risk of engaging in unprotected receptive anal intercourse when the exposure to transphobia is high. Therefore, as per the study shows a correlation between transphobia and high risk of HIV.

Transphobia is a range of negative attitudes, feelings or actions toward transgender or transsexual people, or toward transsexuality. Transphobia can be emotional disgust, fear, violence, anger or discomfort felt or expressed towards people who do not conform to society's gender expectations. It is often expressed alongside homophobic views and hence is often considered an aspect of homophobia. Transphobia is a type of prejudice and discrimination similar to racism and sexism, and transgender people of color are often subjected to all three forms of discrimination at once.

Child victims of transphobia experience harassment, school bullying, and violence in school, foster care, and social programs. Adult victims experience public ridicule, harassment including misgendering, taunts, threats of violence, robbery, and false arrest; many feel unsafe in public. A high percentage report being victims of sexual violence. Some are refused healthcare or suffer workplace discrimination, including being fired for being transgender, or feel under siege by conservative political or religious groups who oppose laws to protect them. There is even discrimination from some people within the movement for the rights of gender and sexual minorities.

Besides the increased risk of violence and other threats, the stress created by transphobia can cause negative emotional consequences which may lead to substance abuse, running away from home (in minors), and a higher rate of suicide.

In the Western world, there have been gradual changes towards the establishment of policies of non-discrimination and equal opportunity. The trend is also taking shape in developing nations. In addition, campaigns regarding the LGBT community are being spread around the world to improve acceptance; the "Stop the Stigma" campaign by the UN is one such development.

Homophobia encompasses a range of negative attitudes and feelings toward homosexuality or people who are identified or perceived as being lesbian, gay, bisexual or transgender (LGBT). It has been defined as contempt, prejudice, aversion, hatred or antipathy, may be based on irrational fear, and is often related to religious beliefs.

Homophobia is observable in critical and hostile behavior such as discrimination and violence on the basis of sexual orientations that are non-heterosexual. Recognized types of homophobia include "institutionalized" homophobia, e.g. religious homophobia and state-sponsored homophobia, and "internalized" homophobia, experienced by people who have same-sex attractions, regardless of how they identify.

Negative attitudes toward identifiable LGBT groups have similar yet specific names: lesbophobia is the intersection of homophobia and sexism directed against lesbians, biphobia targets bisexuality and bisexual people, and transphobia targets transgender and transsexual people and gender variance or gender role nonconformity. According to 2010 Hate Crimes Statistics released by the FBI National Press Office, 19.3 percent of hate crimes across the United States "were motivated by a sexual orientation bias." Moreover, in a Southern Poverty Law Center 2010 "Intelligence Report" extrapolating data from fourteen years (1995–2008), which had complete data available at the time, of the FBI's national hate crime statistics found that LGBT people were "far more likely than any other minority group in the United States to be victimized by violent hate crime."

The term "homophobia" and its usage have been criticized by several sources as unwarrantedly pejorative

While some people use only the more general term "homophobia" to describe this sort of prejudice or behavior, others believe that the terms "homosexual" and "homophobia" do not adequately reflect the specific concerns of lesbians, because they experience the double discrimination of both homophobia and sexism. Similarly, bisexual women may prefer to use the term biphobia to refer to prejudice or abuse that they encounter which is based on their bisexual identity or behaviour, and people who identify as transgender often prefer to use the word transphobia.

Many stereotypes about people who identify as bisexual stem from denial or bisexual erasure. Because their orientation is not recognized as valid, they are stereotyped as confused, indecisive, insecure, experimenting, or "just going through a phase".

The association of bisexuality with promiscuity stems from a variety of negative stereotypes targeting bisexuals as mentally or socially unstable people for whom sexual relations only with men, only with women, or only with one person at a time is not enough. These stereotypes may result from cultural assumptions that "men and women are so different that desire for one is an entirely different beast from desire for the other" ("a defining feature of heterosexism"), and that "verbalizing a sexual desire inevitably leads to attempts to satisfy that desire."

As a result, bisexuals bear a social stigma from accusations of cheating on or betraying their partners, leading a double life, being "on the down-low", and spreading sexually transmitted diseases such as HIV/AIDS. This presumed behavior is further generalized as dishonesty, secrecy, and deception. Bisexuals can be characterized as being "slutty", "easy", indiscriminate, and nymphomaniacs. Furthermore, they are strongly associated with polyamory, swinging, and polygamy, the last being an established heterosexual tradition sanctioned by some religions and legal in several countries. This is despite the fact that bisexual people are as capable of monogamy or serial monogamy as homosexuals or heterosexuals.

Internalized homophobia refers to negative stereotypes, beliefs, stigma, and prejudice about homosexuality and LGBT people that a person with same-sex attraction turns inward on themselves, whether or not they identify as LGBT. The degree to which someone is affected by these ideas depends on how much and which ideas they have consciously and subconsciously internalized. These negative beliefs can be mitigated with education, life experience and therapy, especially with gay-friendly psychotherapy/analysis. Internalized homophobia also applies to conscious or unconscious behaviors which a person feels the need to promote or conform to cultural expectations of heteronormativity or heterosexism. This can include extreme repression and denial coupled with forced outward displays of heteronormative behavior for the purpose of appearing or attempting to feel "normal" or "accepted." Other expressions of internalized homophobia can also be subtle. Some less overt behaviors may include making assumptions about the gender of a person's romantic partner, or about gender roles. Some researchers also apply this label to LGBT people who support "compromise" policies, such as those that find civil unions acceptable in place of same-sex marriage.

Some studies have shown that people who are homophobic are more likely to have repressed homosexual desires. In 1996, a controlled study of 64 heterosexual men (half said they were homophobic by experience, with self-reported orientation) at the University of Georgia found that men who were found to be homophobic (as measured by the Index of Homophobia) were considerably more likely to experience more erectile responses when exposed to homoerotic images than non-homophobic men. Another study in 2012 arrived at similar results when researchers found that students who came from "the most rigid anti-gay homes" were most likely to reveal repressed homosexual attraction. The researchers said that this explained why some religious leaders who denounce homosexuality are later revealed to have secret homosexual relations. They noted that "these people are at war with themselves and are turning this internal conflict outward." A 2016 eye-tracking study showed that heterosexual men with high negative impulse reactions toward homosexuals gazed for longer periods at homosexual imagery than other heterosexual men.

Researcher Iain R. Williamson, in his 1998 paper "Internalized Homophobia and Health Issues Affecting Lesbians and Gay Men" finds the term "homophobia" to be "highly problematic" but for reasons of continuity and consistency with the majority of other publications on the issue retains its use rather than using more accurate but obscure terminology. The phrase "internalized sexual stigma" is sometimes used in place to represent internalized homophobia. An internalized stigma arises when a person believes negative stereotypes about themselves, regardless of where the stereotypes come from. It can also refer to many stereotypes beyond sexuality and gender roles. Internalized homophobia can cause discomfort with and disapproval of one's own sexual orientation. Ego-dystonic sexual orientation or egodystonic homophobia, for instance, is a condition characterized by having a sexual orientation or an attraction that is at odds with one's idealized self-image, causing anxiety and a desire to change one's orientation or become more comfortable with one's sexual orientation. Such a situation may cause extreme repression of homosexual desires. In other cases, a conscious internal struggle may occur for some time, often pitting deeply held religious or social beliefs against strong sexual and emotional desires. This discordance can cause clinical depression, and a higher rate of suicide among LGBT youth (up to 30 percent of non-heterosexual youth attempt suicide) has been attributed to this phenomenon. Psychotherapy, such as gay affirmative psychotherapy, and participation in a sexual-minority affirming group can help resolve the internal conflicts, such as between religious beliefs and sexual identity. Even informal therapies that address understanding and accepting of non-heterosexual orientations can prove effective. Many diagnostic "Internalized Homophobia Scales" can be used to measure a person's discomfort with their sexuality and some can be used by people regardless of gender or sexual orientation. Critics of the scales note that they presume a discomfort with non-heterosexuality which in itself enforces heternormativity.